Journal of Bone and Joint Infection
Journal of Bone and Joint Infection
JBJI
Articles | Volume 9, issue 1
Articles | Volume 9, issue 1
https://doi.org/10.5194/jbji-9-37-2024
© Author(s) 2024. This work is distributed under
the Creative Commons Attribution 4.0 License.
https://doi.org/10.5194/jbji-9-37-2024
© Author(s) 2024. This work is distributed under
the Creative Commons Attribution 4.0 License.
Articles | Volume 9, issue 1
Original full-length article
 | 
06 Feb 2024
Original full-length article |  | 06 Feb 2024

Optimized decision algorithm for the microbiological diagnosis of osteoarticular infections in adults using synovial fluid samples: a prospective study in two French hospitals including 423 samples of synovial fluid

Céline Dupieux, Ghislaine Descours, Paul Verhoeven, Florence Grattard, Yvonne Benito, François Vandenesch, Céline Cazorla, Tristan Ferry, Sébastien Lustig, Bertrand Boyer, Sandrine Boisset, Anne Carricajo, Frédéric Laurent, and PIRLA investigator group

Related authors

Debridement, antimicrobial therapy, and implant retention (DAIR) as curative surgical strategy for acute periprosthetic hip and knee infections: a summary of the position paper from the European Bone & Joint Infection Society (EBJIS)
Irene K. Sigmund, Marjan Wouthuyzen-Bakker, Tristan Ferry, Willem J. Metsemakers, Martin Clauss, Alex Soriano, Rihard Trebse, and Ricardo Sousa
J. Bone Joint Infect., 10, 139–142, https://doi.org/10.5194/jbji-10-139-2025,https://doi.org/10.5194/jbji-10-139-2025, 2025
Short summary
Debridement, antimicrobial therapy, and implant retention (DAIR) as curative strategy for acute periprosthetic hip and knee infections: a position paper of the European Bone & Joint Infection Society (EBJIS)
Irene K. Sigmund, Tristan Ferry, Ricardo Sousa, Alex Soriano, Willem J. Metsemakers, Martin Clauss, Rihard Trebse, and Marjan Wouthuyzen-Bakker
J. Bone Joint Infect., 10, 101–138, https://doi.org/10.5194/jbji-10-101-2025,https://doi.org/10.5194/jbji-10-101-2025, 2025
Short summary
Potential value of a rapid syndromic multiplex PCR for the diagnosis of native and prosthetic joint infections: a real-world evidence study
Stéphanie Pascual, Brooklyn Noble, Nusreen Ahmad-Saeed, Catherine Aldridge, Simone Ambretti, Sharon Amit, Rachel Annett, Shaan Ashk O'Shea, Anna Maria Barbui, Gavin Barlow, Lucinda Barrett, Mario Berth, Alessandro Bondi, Nicola Boran, Sara E. Boyd, Catarina Chaves, Martin Clauss, Peter Davies, Ileana T. Dianzo-Delgado, Jaime Esteban, Stefan Fuchs, Lennart Friis-Hansen, Daniel Goldenberger, Andrej Kraševac Glaser, Juha O. Groonroos, Ines Hoffmann, Tomer Hoffmann, Harriet Hughes, Marina Ivanova, Peter Jezek, Gwennan Jones, Zeynep Ceren Karahan, Cornelia Lass-Flörl, Frédéric Laurent, Laura Leach, Matilde Lee Horsbøll Pedersen, Caroline Loiez, Maureen Lynch, Robert J. Maloney, Martin Marsh, Olivia Milburn, Shanine Mitchell, Luke S. P. Moore, Lynn Moffat, Marianna Murdjeva, Michael E. Murphy, Deepa Nayar, Giacomo Nigrisoli, Fionnuala O'Sullivan, Büşra Öz, Teresa Peach, Christina Petridou, Mojgan Prinz, Mitja Rak, Niamh Reidy, Gian Maria Rossolini, Anne-Laure Roux, Patricia Ruiz-Garbajosa, Kordo Saeed, Llanos Salar-Vidal, Carlos Salas Venero, Mathyruban Selvaratnam, Eric Senneville, Peter Starzengruber, Ben Talbot, Vanessa Taylor, Rihard Trebše, Deborah Wearmouth, Birgit Willinger, Marjan Wouthuyzen-Bakker, Brianne Couturier, and Florence Allantaz
J. Bone Joint Infect., 9, 87–97, https://doi.org/10.5194/jbji-9-87-2024,https://doi.org/10.5194/jbji-9-87-2024, 2024
Short summary
Lysins – a new armamentarium for the treatment of bone and joint infections?
Parham Sendi and Tristan Ferry
J. Bone Joint Infect., 7, 187–189, https://doi.org/10.5194/jbji-7-187-2022,https://doi.org/10.5194/jbji-7-187-2022, 2022
Osteocutaneous-flap-related osteomyelitis following mandibular reconstruction: a cohort study of an emerging and complex bone infection
Clément Javaux, Clémentine Daveau, Clotilde Bettinger, Mathieu Daurade, Céline Dupieux-Chabert, Fabien Craighero, Carine Fuchsmann, Philippe Céruse, Arnaud Gleizal, Nicolas Sigaux, Tristan Ferry, Florent Valour, and the Lyon BJI study group
J. Bone Joint Infect., 7, 127–136, https://doi.org/10.5194/jbji-7-127-2022,https://doi.org/10.5194/jbji-7-127-2022, 2022
Short summary
More articles (2)

Related subject area

Subject: Microbiology of bone and joint infections | Topic: All topics
Multi-centre evaluation of Gram stain in the diagnosis of septic arthritis
Charlotte Smith, Robert J. Maloney, Deborah Wearmouth, Hemant Sharma, Kordo Saeed, Nusreen Ahmad-Saeed, Rachel Annett, Lucinda Barrett, Sara E. Boyd, Peter Davies, Harriet Hughes, Gwennan Jones, Laura Leach, Maureen Lynch, Deepa Nayar, Martin Marsh, Shanine Mitchell, Lynn Moffat, Luke S. P. Moore, Michael E. Murphy, Shaan Ashk O'Shea, Teresa Peach, Christina Petridou, Niamh Reidy, Ben Talbot, Catherine Aldridge, and Gavin Barlow
J. Bone Joint Infect., 10, 61–71, https://doi.org/10.5194/jbji-10-61-2025,https://doi.org/10.5194/jbji-10-61-2025, 2025
Short summary
Yield of routine mycobacterial culture of osteoarticular specimens in a tertiary orthopaedic hospital in England, 2017–2022
Tom A. Yates, Olivier Vahesan, Simon Warren, and Antonia Scobie
J. Bone Joint Infect., 9, 217–223, https://doi.org/10.5194/jbji-9-217-2024,https://doi.org/10.5194/jbji-9-217-2024, 2024
Short summary
Musculoskeletal infections associated with Nocardia species: a case series
Ryan B. Khodadadi, Jack W. McHugh, Supavit Chesdachai, Nancy L. Wengenack, Wendelyn Bosch, Maria Teresa Seville, Douglas R. Osmon, Elena Beam, and Zachary A. Yetmar
J. Bone Joint Infect., 9, 207–212, https://doi.org/10.5194/jbji-9-207-2024,https://doi.org/10.5194/jbji-9-207-2024, 2024
Short summary
Musculoskeletal manifestations of lower-extremity coccidioidomycosis: a case series
William Estes, L. Daniel Latt, Jacob Robishaw-Denton, Matthew L. Repp, Yash Suri, Tyson Chadaz, Christina Boulton, and Talha Riaz
J. Bone Joint Infect., 9, 197–205, https://doi.org/10.5194/jbji-9-197-2024,https://doi.org/10.5194/jbji-9-197-2024, 2024
Short summary
Potential value of a rapid syndromic multiplex PCR for the diagnosis of native and prosthetic joint infections: a real-world evidence study
Stéphanie Pascual, Brooklyn Noble, Nusreen Ahmad-Saeed, Catherine Aldridge, Simone Ambretti, Sharon Amit, Rachel Annett, Shaan Ashk O'Shea, Anna Maria Barbui, Gavin Barlow, Lucinda Barrett, Mario Berth, Alessandro Bondi, Nicola Boran, Sara E. Boyd, Catarina Chaves, Martin Clauss, Peter Davies, Ileana T. Dianzo-Delgado, Jaime Esteban, Stefan Fuchs, Lennart Friis-Hansen, Daniel Goldenberger, Andrej Kraševac Glaser, Juha O. Groonroos, Ines Hoffmann, Tomer Hoffmann, Harriet Hughes, Marina Ivanova, Peter Jezek, Gwennan Jones, Zeynep Ceren Karahan, Cornelia Lass-Flörl, Frédéric Laurent, Laura Leach, Matilde Lee Horsbøll Pedersen, Caroline Loiez, Maureen Lynch, Robert J. Maloney, Martin Marsh, Olivia Milburn, Shanine Mitchell, Luke S. P. Moore, Lynn Moffat, Marianna Murdjeva, Michael E. Murphy, Deepa Nayar, Giacomo Nigrisoli, Fionnuala O'Sullivan, Büşra Öz, Teresa Peach, Christina Petridou, Mojgan Prinz, Mitja Rak, Niamh Reidy, Gian Maria Rossolini, Anne-Laure Roux, Patricia Ruiz-Garbajosa, Kordo Saeed, Llanos Salar-Vidal, Carlos Salas Venero, Mathyruban Selvaratnam, Eric Senneville, Peter Starzengruber, Ben Talbot, Vanessa Taylor, Rihard Trebše, Deborah Wearmouth, Birgit Willinger, Marjan Wouthuyzen-Bakker, Brianne Couturier, and Florence Allantaz
J. Bone Joint Infect., 9, 87–97, https://doi.org/10.5194/jbji-9-87-2024,https://doi.org/10.5194/jbji-9-87-2024, 2024
Short summary
More articles (3)

Cited articles

Bauer, H. M., Ting, Y., Greer, C. E., Chambers, J. C., Tashiro, C. J., Chimera, J., Reingold, A., and Manos, M. M.: Genital human papillomavirus infection in female university students as determined by a PCR-based method, JAMA, 265, 472–477, 1991. 
Bémer, P., Plouzeau, C., Tande, D., Léger, J., Giraudeau, B., Valentin, A. S., Jolivet-Gougeon, A., Vincent, P., Corvec, S., Gibaud, S., Juvin, M. E., Héry-Arnaud, G., Lemarié, C., Kempf, M., Bret, L., Quentin, R., Coffre, C., de Pinieux, G., Bernard, L., Burucoa, C., and Centre de Référence des Infections Ostéo-articulaires du Grand Ouest (CRIOGO) Study Team: Evaluation of 16S rRNA gene PCR sensitivity and specificity for diagnosis of prosthetic joint infection: a prospective multicenter cross-sectional study, J. Clin. Microbiol., 52, 3583–3589, https://doi.org/10.1128/JCM.01459-14, 2014. 
Bémer, P., Léger, J., Tandé, D., Plouzeau, C., Valentin, A. S., Jolivet-Gougeon, A., Lemarié, C., Kempf, M., Héry-Arnaud, G., Bret, L., Juvin, M. E., Giraudeau, B., Corvec, S., Burucoa, C., and Centre de Référence des Infections Ostéo-articulaires du Grand Ouest (CRIOGO) Study Team: How many samples and how many culture media to diagnose a prosthetic joint infection: a clinical and microbiological prospective multicenter study, J. Clin. Microbiol., 54, 385–391, https://doi.org/10.1128/JCM.02497-15, 2016. 
Carvalho, M. da G. S., Tondella, M. L., McCaustland, K., Weidlich, L., McGee, L., Mayer, L. W., Steigerwalt, A., Whaley, M., Facklam, R. R., Fields, B., Carlone, G., Ades, E. W., Dagan, R., and Sampson, J. S.: Evaluation and improvement of real-time PCR assays targeting lytA, ply, and psaA genes for detection of pneumococcal DNA, J. Clin. Microbiol., 45, 2460–2466, https://doi.org/10.1128/JCM.02498-06, 2007. 
Cherkaoui, A., Ceroni, D., Emonet, S., Lefevre, Y., and Schrenzel, J.: Molecular diagnosis of Kingella kingae osteoarticular infections by specific real-time PCR assay, J. Med. Microbiol., 58, 65–68, https://doi.org/10.1099/jmm.0.47707-0, 2009. 
More articles (19)
Download
Short summary
We define an algorithm to optimize bone and joint infection diagnosis in adults on synovial fluid samples. This prospective multi-center study included a large number of synovial fluids and compared the performance of classical culture on different media, inoculation of synovial fluid in blood culture bottles, and broad-range and specific PCR. We proposed an algorithm associating culture onto three solid media and into blood culture bottles and 16S, Staphylococcus, and Streptococcus PCR.
Read more
Share