Articles | Volume 8, issue 2
https://doi.org/10.5194/jbji-8-81-2023
https://doi.org/10.5194/jbji-8-81-2023
Original full-length article
 | 
07 Mar 2023
Original full-length article |  | 07 Mar 2023

Murine models of orthopedic infection featuring Staphylococcus aureus biofilm

Aiken Dao, Alexandra K. O'Donohue, Emily R. Vasiljevski, Justin D. Bobyn, David G. Little, and Aaron Schindeler

Related subject area

Subject: Osteomyelitis | Topic: Other
A natural history of untreated chronic osteomyelitis of the tibia over 20 years, with evolving squamous cell carcinoma: a case report
Asanka Wijendra, Alex Ramsden, and Martin McNally
J. Bone Joint Infect., 8, 183–188, https://doi.org/10.5194/jbji-8-183-2023,https://doi.org/10.5194/jbji-8-183-2023, 2023
Short summary
Osteocutaneous-flap-related osteomyelitis following mandibular reconstruction: a cohort study of an emerging and complex bone infection
Clément Javaux, Clémentine Daveau, Clotilde Bettinger, Mathieu Daurade, Céline Dupieux-Chabert, Fabien Craighero, Carine Fuchsmann, Philippe Céruse, Arnaud Gleizal, Nicolas Sigaux, Tristan Ferry, Florent Valour, and the Lyon BJI study group
J. Bone Joint Infect., 7, 127–136, https://doi.org/10.5194/jbji-7-127-2022,https://doi.org/10.5194/jbji-7-127-2022, 2022
Short summary

Cited articles

Alstrup, A. K. O., Jensen, S. B., Nielsen, O. L., Jødal, L., and Afzelius, P.: Preclinical Testing of Radiopharmaceuticals for the Detection and Characterization of Osteomyelitis: Experiences from a Porcine Model, Molecules, 26, 4221, https://doi.org/10.3390/molecules26144221, 2021. 
Billings, C. and Anderson, D. E.: Role of Animal Models to Advance Research of Bacterial Osteomyelitis, Front. Vet. Sci., 9, 879630, https://doi.org/10.3389/fvets.2022.879630, 2022. 
Boot, W., Schmid, T., D'Este, M., Guillaume, O., Foster, A., Decosterd, L., Richards, R. G., Eglin, D., Zeiter, S., and Moriarty, T. F.: A Hyaluronic Acid Hydrogel Loaded with Gentamicin and Vancomycin Successfully Eradicates Chronic Methicillin-Resistant Staphylococcus aureus Orthopedic Infection in a Sheep Model, Antimicrob. Agents Ch., 65, e01840-20, https://doi.org/10.1128/aac.01840-20, 2021. 
Brinkman, C. L., Schmidt-Malan, S. M., Karau, M. J., and Patel, R.: A novel rat model of foreign body osteomyelitis for evaluation of antimicrobial efficacy, J. Exp. Appl. Anim. Sci., 3, 7–14, https://doi.org/10.20454/jeaas.2019.1555, 2019. 
Cassano, P., Ciprandi, G., and Passali, D.: Acute mastoiditis in children, Acta Biomed., 91, 54–59, https://doi.org/10.23750/abm.v91i1-S.9259, 2020. 
Download
Short summary
Bone infections are challenging to treat and require new treatments. The current animal models for testing new therapies can be expensive and complex; therefore, we developed a cheap and reliable bone infection model in mice. Our new model features an infected hole created by a needle in the leg bone with a metal pin. This method achieved a consistent infection rate and demonstrates the importance of bacterial biofilm in bone infection.