Articles | Volume 8, issue 2
https://doi.org/10.5194/jbji-8-81-2023
https://doi.org/10.5194/jbji-8-81-2023
Original full-length article
 | 
07 Mar 2023
Original full-length article |  | 07 Mar 2023

Murine models of orthopedic infection featuring Staphylococcus aureus biofilm

Aiken Dao, Alexandra K. O'Donohue, Emily R. Vasiljevski, Justin D. Bobyn, David G. Little, and Aaron Schindeler

Video supplement

Demonstration of the needle insertion surgical technique Aiken Dao https://cloudstor.aarnet.edu.au/plus/s/lpMUFHeb9RUsw1a

Demonstration of the drilled hole surgical technique Aiken Dao https://cloudstor.aarnet.edu.au/plus/s/ybi3XzdoSKMSnMz

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Short summary
Bone infections are challenging to treat and require new treatments. The current animal models for testing new therapies can be expensive and complex; therefore, we developed a cheap and reliable bone infection model in mice. Our new model features an infected hole created by a needle in the leg bone with a metal pin. This method achieved a consistent infection rate and demonstrates the importance of bacterial biofilm in bone infection.