Journal of Bone and Joint Infection
Journal of Bone and Joint Infection
JBJI
Articles | Volume 8, issue 1
Articles | Volume 8, issue 1
https://doi.org/10.5194/jbji-8-45-2023
© Author(s) 2023. This work is distributed under
the Creative Commons Attribution 4.0 License.
https://doi.org/10.5194/jbji-8-45-2023
© Author(s) 2023. This work is distributed under
the Creative Commons Attribution 4.0 License.
Articles | Volume 8, issue 1
Original full-length article
 | 
18 Jan 2023
Original full-length article |  | 18 Jan 2023

First evaluation of a commercial multiplex PCR panel for rapid detection of pathogens associated with acute joint infections

Jorrit Willem Adriaan Schoenmakers, Rosanne de Boer, Lilli Gard, Greetje Anna Kampinga, Marleen van Oosten, Jan Maarten van Dijl, Paulus Christiaan Jutte, and Marjan Wouthuyzen-Bakker

Related authors

Debridement, antimicrobial therapy, and implant retention (DAIR) as curative surgical strategy for acute periprosthetic hip and knee infections: a summary of the position paper from the European Bone & Joint Infection Society (EBJIS)
Irene K. Sigmund, Marjan Wouthuyzen-Bakker, Tristan Ferry, Willem-Jan Metsemakers, Martin Clauss, Alex Soriano, Rihard Trebse, and Ricardo Sousa
J. Bone Joint Infect., 10, 139–142, https://doi.org/10.5194/jbji-10-139-2025,https://doi.org/10.5194/jbji-10-139-2025, 2025
Short summary
Debridement, antimicrobial therapy, and implant retention (DAIR) as curative strategy for acute periprosthetic hip and knee infections: a position paper of the European Bone & Joint Infection Society (EBJIS)
Irene K. Sigmund, Tristan Ferry, Ricardo Sousa, Alex Soriano, Willem-Jan Metsemakers, Martin Clauss, Rihard Trebse, and Marjan Wouthuyzen-Bakker
J. Bone Joint Infect., 10, 101–138, https://doi.org/10.5194/jbji-10-101-2025,https://doi.org/10.5194/jbji-10-101-2025, 2025
Short summary
Postoperative antibiotic treatment does not lower re-revision rate in presumed aseptic hip and knee revision arthroplasties with unexpected positive intraoperative cultures – a matched cohort study
Sebastian Simon, Marjan Wouthuyzen-Bakker, Susana Gardete Hartmann, Jennyfer A. Mitterer, Sujeesh Sebastian, Stephanie Huber, Bernhard J. H. Frank, and Jochen G. Hofstaetter
J. Bone Joint Infect., 10, 51–59, https://doi.org/10.5194/jbji-10-51-2025,https://doi.org/10.5194/jbji-10-51-2025, 2025
Short summary
Predicting periprosthetic joint infection: external validation of preoperative prediction models
Seung-Jae Yoon, Paul C. Jutte, Alex Soriano, Ricardo Sousa, Wierd P. Zijlstra, and Marjan Wouthuyzen-Bakker
J. Bone Joint Infect., 9, 231–239, https://doi.org/10.5194/jbji-9-231-2024,https://doi.org/10.5194/jbji-9-231-2024, 2024
Short summary
Potential value of a rapid syndromic multiplex PCR for the diagnosis of native and prosthetic joint infections: a real-world evidence study
Stéphanie Pascual, Brooklyn Noble, Nusreen Ahmad-Saeed, Catherine Aldridge, Simone Ambretti, Sharon Amit, Rachel Annett, Shaan Ashk O'Shea, Anna Maria Barbui, Gavin Barlow, Lucinda Barrett, Mario Berth, Alessandro Bondi, Nicola Boran, Sara E. Boyd, Catarina Chaves, Martin Clauss, Peter Davies, Ileana T. Dianzo-Delgado, Jaime Esteban, Stefan Fuchs, Lennart Friis-Hansen, Daniel Goldenberger, Andrej Kraševac Glaser, Juha O. Groonroos, Ines Hoffmann, Tomer Hoffmann, Harriet Hughes, Marina Ivanova, Peter Jezek, Gwennan Jones, Zeynep Ceren Karahan, Cornelia Lass-Flörl, Frédéric Laurent, Laura Leach, Matilde Lee Horsbøll Pedersen, Caroline Loiez, Maureen Lynch, Robert J. Maloney, Martin Marsh, Olivia Milburn, Shanine Mitchell, Luke S. P. Moore, Lynn Moffat, Marianna Murdjeva, Michael E. Murphy, Deepa Nayar, Giacomo Nigrisoli, Fionnuala O'Sullivan, Büşra Öz, Teresa Peach, Christina Petridou, Mojgan Prinz, Mitja Rak, Niamh Reidy, Gian Maria Rossolini, Anne-Laure Roux, Patricia Ruiz-Garbajosa, Kordo Saeed, Llanos Salar-Vidal, Carlos Salas Venero, Mathyruban Selvaratnam, Eric Senneville, Peter Starzengruber, Ben Talbot, Vanessa Taylor, Rihard Trebše, Deborah Wearmouth, Birgit Willinger, Marjan Wouthuyzen-Bakker, Brianne Couturier, and Florence Allantaz
J. Bone Joint Infect., 9, 87–97, https://doi.org/10.5194/jbji-9-87-2024,https://doi.org/10.5194/jbji-9-87-2024, 2024
Short summary
More articles (6)

Related subject area

Subject: Prosthesis-related infections | Topic: Other
Synovial glucose and serum-to-synovial-glucose ratio perform better than other biomarkers for the diagnosis of acute postoperative prosthetic knee infection
Marta Sabater-Martos, Oscar Garcia, Laia Boadas, Laura Morata, Alex Soriano, and Juan Carlos Martínez-Pastor
J. Bone Joint Infect., 10, 41–49, https://doi.org/10.5194/jbji-10-41-2025,https://doi.org/10.5194/jbji-10-41-2025, 2025
Short summary
Changing the definition of treatment success alters treatment outcomes in periprosthetic joint infection: a systematic review and meta-analysis
Eytan M. Debbi, Tyler Khilnani, Ioannis Gkiatas, Yu-Fen Chiu, Andy O. Miller, Michael W. Henry, and Alberto V. Carli
J. Bone Joint Infect., 9, 127–136, https://doi.org/10.5194/jbji-9-127-2024,https://doi.org/10.5194/jbji-9-127-2024, 2024
Short summary
Diagnostic cutoff values of synovial fluid biomarkers for acute postoperative prosthetic joint infection: a systematic review and meta-analysis
Marta Sabater-Martos, Marc Ferrer, Laura Morata, Alex Soriano, and Juan Carlos Martínez-Pastor
J. Bone Joint Infect., 9, 17–26, https://doi.org/10.5194/jbji-9-17-2024,https://doi.org/10.5194/jbji-9-17-2024, 2024
Short summary
Is ultrasound-guided hip aspiration more successful than fluoroscopic-guided aspiration in diagnosing prosthetic joint infection?
Emily A. Treu, Daniel M. Cushman, John C. Wheelwright, Brenna E. Blackburn, Masaru Teramoto, and Michael J. Archibeck
J. Bone Joint Infect., 8, 151–163, https://doi.org/10.5194/jbji-8-151-2023,https://doi.org/10.5194/jbji-8-151-2023, 2023
Short summary
Simple and inexpensive synovial fluid biomarkers for the diagnosis of prosthetic joint infection according to the new EBJIS definition
Sara Elisa Diniz, Ana Ribau, André Vinha, José Carlos Oliveira, Miguel Araújo Abreu, and Ricardo Sousa
J. Bone Joint Infect., 8, 109–118, https://doi.org/10.5194/jbji-8-109-2023,https://doi.org/10.5194/jbji-8-109-2023, 2023
Short summary
More articles (3)

Cited articles

Arvieux, C. and Common, H.: New diagnostic tools for prosthetic joint infection, Orthop. Traumatol. Surg. Res., 105, S23–S30, https://doi.org/10.1016/j.otsr.2018.04.029, 2019. 
Benito, N., Mur, I., Ribera, A., Soriano, A., Rodríguez-Pardo, D., Sorlí, L., Cobo, J., Fernández-Sampedro, M., Del Toro, M. D., Guío, L., Praena, J., Bahamonde, A., Riera, M., Esteban, J., Baraia-Etxaburu, J. M., Martínez-Alvarez, J., Jover-Sáenz, A., Dueñas, C., Ramos, A., Sobrino, B., Euba, G., Morata, L., Pigrau, C., Horcajada, J. P., Coll, P., Crusi, X., Ariza, J., REIPI (Spanish Network for Research in Infectious Disease) Group for the Study of Prosthetic Joint Infections / GEIO (Group for the Study of Osteoarticular Infections), and SEIMC (Spanish Society of Infectious Diseases and Clinical Microbiolo: The Different Microbial Etiology of Prosthetic Joint Infections according to Route of Acquisition and Time after Prosthesis Implantation, Including the Role of Multidrug-Resistant Organisms, J. Clin. Med., 8, 673, https://doi.org/10.3390/jcm8050673, 2019. 
Berneking, L., Haas, M., Frielinghaus, L., Berinson, B., Lütgehetmann, M., Christner, M., Aepfelbacher, M., Gerlach, U., Seide, K., Both, A., and Rohde, H.: Evaluation of a syndromic panel polymerase chain reaction (spPCR) assay for the diagnosis of device-associated bone and joint infections (BJI), Int. J. Infect. Dis., 116, 283–288, https://doi.org/10.1016/j.ijid.2022.01.013, 2022. 
Cunha, C. B. and Opal, S. M.: Antibiotic Stewardship: Strategies to Minimize Antibiotic Resistance While Maximizing Antibiotic Effectiveness, Med. Clin. North Am., 102, 831–843, https://doi.org/10.1016/j.mcna.2018.04.006, 2018. 
Fye, K.: Arthrocentesis, synovial fluid analysis, and synovial biopsy, in: Primer on the Rheumatic Diseases, edited by: Klippel, J. H., 13th edn., Atlanta, USA, 2008. 
More articles (15)
Download
Short summary
In a first evaluation, the accuracy of a novel multiplex PCR (polymerase chain reaction) panel for rapid detection of pathogens in patients with a clinical suspicion of acute septic arthritis of native and prosthetic joints is assessed. Clear benefit is seen in patients with a suspected native septic arthritis and late acute prosthetic joint infection. This indicates that the panel allows for fast tailoring of antibiotics and may prompt the surgeon for surgical lavage in doubtful clinical cases.
Read more
Share