Articles | Volume 8, issue 1
https://doi.org/10.5194/jbji-8-45-2023
© Author(s) 2023. This work is distributed under
the Creative Commons Attribution 4.0 License.
the Creative Commons Attribution 4.0 License.
https://doi.org/10.5194/jbji-8-45-2023
© Author(s) 2023. This work is distributed under
the Creative Commons Attribution 4.0 License.
the Creative Commons Attribution 4.0 License.
First evaluation of a commercial multiplex PCR panel for rapid detection of pathogens associated with acute joint infections
Jorrit Willem Adriaan Schoenmakers
Department of Orthopaedic Surgery, University of Groningen,
University Medical Center Groningen (UMCG), Groningen, The Netherlands
Department of Medical Microbiology and Infection Prevention,
University of Groningen, UMCG, Groningen, The Netherlands
Rosanne de Boer
Department of Medical Microbiology and Infection Prevention,
University of Groningen, UMCG, Groningen, The Netherlands
Lilli Gard
Department of Medical Microbiology and Infection Prevention,
University of Groningen, UMCG, Groningen, The Netherlands
Greetje Anna Kampinga
Department of Medical Microbiology and Infection Prevention,
University of Groningen, UMCG, Groningen, The Netherlands
Marleen van Oosten
Department of Medical Microbiology and Infection Prevention,
University of Groningen, UMCG, Groningen, The Netherlands
Jan Maarten van Dijl
Department of Medical Microbiology and Infection Prevention,
University of Groningen, UMCG, Groningen, The Netherlands
Paulus Christiaan Jutte
Department of Orthopaedic Surgery, University of Groningen,
University Medical Center Groningen (UMCG), Groningen, The Netherlands
Marjan Wouthuyzen-Bakker
CORRESPONDING AUTHOR
Department of Medical Microbiology and Infection Prevention,
University of Groningen, UMCG, Groningen, The Netherlands
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Cited
33 citations as recorded by crossref.
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- From serum inflammatory markers to fluid, tissue, and molecular assays: current advances in the laboratory diagnosis of bone and joint infections J. Li et al. https://doi.org/10.3389/fcimb.2026.1865643
- Culture-negative periprosthetic joint infection: an umbrella review of prevalence, diagnostic strategies, treatment, and methodological limitations G. Bocchino et al. https://doi.org/10.5194/jbji-11-479-2026
- Recent updates in the development of molecular assays for the rapid identification and susceptibility testing of MRSA M. Mizusawa & K. Carroll https://doi.org/10.1080/14737159.2023.2234823
- Evaluation of the BioFire joint infection panel in Taiwan: Impact of regional non-target pathogens and joint type on its performance S. Lo et al. https://doi.org/10.1016/j.jmii.2026.10.004
- Multicenter evaluation of BioFire JI panel related to improved microbiological diagnostics on acute osteoarticular infections L. Salar-Vidal et al. https://doi.org/10.1016/j.ijmm.2023.151588
- Diagnosis and Treatment of Acute Periprosthetic Infections with the BioFire® System within a Time-Dependent and Bacterium-Dependent Protocol: Review and Prosthesis-Saving Protocol R. Sangaletti et al. https://doi.org/10.3390/biomedicines12092082
- Automated multiplex PCR of BioFire joint infection panel enables rapid pathogen identification in periprosthetic joint infection excluding coagulase-negative Staphylococci H. Choe et al. https://doi.org/10.1016/j.jos.2025.07.008
- Atypical Staphylococcal Septic Arthritis in a Native Hip: A Case Report and Review I. Glassman et al. https://doi.org/10.3390/pathogens12030408
- Microfluidic and lab-on-a-chip devices for detection and diagnosis of periprosthetic joint infections L. Pellegrino et al. https://doi.org/10.1007/s10544-025-00768-9
- High sensitivity and enhanced antibiotic stewardship of the BioFire Joint Infection Panel in acute, but not chronic, prosthetic joint infection of the knee T. Lin et al. https://doi.org/10.1128/spectrum.00286-25
- Mikrobiologische Diagnostik bei Gelenkinfektionen F. Olearo et al. https://doi.org/10.1055/a-2760-6093
- Effect of Preoperative Antibiotic Therapy on Operative Culture Yield for Diagnosis of Native Joint Septic Arthritis R. Khodadadi et al. https://doi.org/10.1093/cid/ciae136
- Beyond Cultures: The Evolving Role of Molecular Diagnostics, Synovial Biomarkers and Artificial Intelligence in the Diagnosis of Prosthetic Joint Infections M. Maritati et al. https://doi.org/10.3390/jcm14196886
- Polymerase Chain Reaction as an Adjunct to Conventional Culture for Periprosthetic Joint Infection Diagnosis A. Sarfraz et al. https://doi.org/10.1016/j.arth.2026.03.059
- State-of-the-Art Review: Native Joint Septic Arthritis Epidemiology, Pathogenesis, Microbiology, Diagnosis, Management, and Outcomes R. Khodadadi et al. https://doi.org/10.1093/cid/ciag473
- A fluorogenic micrococcal nuclease-based probe for fast detection and optical imaging of Staphylococcus aureus in prosthetic joint and fracture-related infections J. Schoenmakers et al. https://doi.org/10.1007/s00259-023-06499-4
- Evaluation of a fully automated multiplex PCR panel for the microbiological diagnosis of joint infections N. Ueda et al. https://doi.org/10.1016/j.jos.2025.12.004
- Rapid and Accurate Diagnosis of Joint Infections H. Jeong et al. https://doi.org/10.2106/JBJS.OA.25.00217
- Sonication protocols and their contributions to the microbiological diagnosis of implant-associated infections: a review of the current scenario N. Silva et al. https://doi.org/10.3389/fcimb.2024.1398461
- Molecular diagnostics for perioperative microbial identification in periprosthetic joint infection: A scoping review and proposal of a diagnostic flow chart P. Indelli et al. https://doi.org/10.1002/jeo2.70263
- Evaluating the Diagnostic Performance of a Multiplex Polymerase Chain Reaction Panel for the Detection of Synovial Fluid Pathogens in the Setting of Periprosthetic Joint Infection J. Miamidian et al. https://doi.org/10.7759/cureus.91300
- Synovial Fluid Analysis in Melioidosis: Experiences from the Darwin Prospective Melioidosis Study S. Campbell et al. https://doi.org/10.3390/pathogens14111120
- Performance and potential utility of the BioFire Joint Infection Panel with synovial fluid and joint tissue specimens S. Fox-Lewis et al. https://doi.org/10.1128/jcm.00594-25
- Prospective evaluation of real-world performance and clinical impact of the Biofire FilmArray joint infection panel B. Berinson et al. https://doi.org/10.1128/spectrum.02239-24
- Clinical comparison of the BIOFIRE Joint Infection Panel using synovial fluid and tissue biopsy samples L. Salar-Vidal et al. https://doi.org/10.1128/spectrum.03402-25
- Evaluation of a BioFire multiplex PCR panel for detection of joint infections using retrospective and prospectively collected specimens A. Moran et al. https://doi.org/10.1128/jcm.00182-24
- Potential value of a rapid syndromic multiplex PCR for the diagnosis of native and prosthetic joint infections: a real-world evidence study S. Pascual et al. https://doi.org/10.5194/jbji-9-87-2024
- The role of BioFire Joint Infection Panel in diagnosing periprosthetic hip and knee joint infections in patients with unclear conventional microbiological results S. Gardete-Hartmann et al. https://doi.org/10.1302/2046-3758.137.BJR-2023-0321.R2
- Clinical evaluation of a multiplex PCR-based test for joint infection: a prospective diagnostic accuracy study of forty-nine patients J. Lund-Andersen et al. https://doi.org/10.1007/s00590-024-04114-2
- BioFire in osteoarticular Infections: Rapid syndromic testing for early and accurate diagnosis – A narrative review V. Viswanathan et al. https://doi.org/10.1016/j.jcot.2025.103038
- Performance of Multiplex Joint Infection Panel in the Diagnosis of Septic Arthritis P. Kesavan et al. https://doi.org/10.1097/IPC.0000000000001407
Latest update: 02 Nov 2026
Short summary
In a first evaluation, the accuracy of a novel multiplex PCR (polymerase chain reaction) panel for rapid detection of pathogens in patients with a clinical suspicion of acute septic arthritis of native and prosthetic joints is assessed. Clear benefit is seen in patients with a suspected native septic arthritis and late acute prosthetic joint infection. This indicates that the panel allows for fast tailoring of antibiotics and may prompt the surgeon for surgical lavage in doubtful clinical cases.
In a first evaluation, the accuracy of a novel multiplex PCR (polymerase chain reaction) panel...