Articles | Volume 9, issue 1
Original full-length article
24 Jan 2024
Original full-length article |  | 24 Jan 2024

Multiplex PCR test as an intra-operative diagnostic tool for periprosthetic joint infection in presumed aseptic revision hip and knee arthroplasty: a 1-year follow-up study of 200 cases

Thomas J. A. van Schaik, Petra J. C. Heesterbeek, Job L. C. van Susante, Wim H. C. Rijnen, and Jon H. M. Goosen

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Cited articles

Aamot, H. V., Johnsen, B. O., and Skråmm, I.: Rapid diagnostics of orthopedic implant-associated infections using Unyvero ITI implant and tissue infection application is not optimal for Staphylococcus species identification, BMC Res. Notes., 12, 725,, 2019. 
Auñón, Á., Coifman, I., Blanco, A., García Cañete, J., Parrón-Cambero, R., and Esteban, J.: Usefulness of a Multiplex PCR Assay for the Diagnosis of Prosthetic Joint Infections in the Routine Setting, Orthop. Surg., 14, 383–388,, 2022. 
Borde, J. P., Häcker, G. A., Guschl, S., Serr, A., Danner, T., Hübner, J., Burrack-Lange, S., Lüdke, G., Helwig, P., Hauschild, O., and Kern, W. V.: Diagnosis of prosthetic joint infections using UMD-Universal Kit and the automated multiplex-PCR Unyvero i60 ITI® cartridge system: a pilot study, Infection, 43, 551–560,, 2015. 
Corvec, S., Portillo, M. E., Pasticci, B. M., Borens, O., and Trampuz, A.: Epidemiology and new developments in the diagnosis of prosthetic joint infection, Int. J. Artif. Organs, 35, 923–934,, 2012. 
Fernandez-Sampedro, M., Salas-Venero, C., Fariñas-Álvarez, C., Sumillera, M., Pérez-Carro, L., Fakkas-Fernandez, M., Gómez-Román, J., Martínez-Martínez, L., and Fariñas, M. C. M.: Postoperative diagnosis and outcome in patients with revision arthroplasty for aseptic loosening, BMC Infect. Dis., 15, 232,, 2015. 
Short summary
This study evaluated the use of an automated custom-made multiplex PCR (mPCR) test of synovial fluid in presumed aseptic revision hip and knee arthroplasties. Of all 200 patients, none developed a periprosthetic joint infection (PJI) within the first year after surgery. The study showed that a positive mPCR test did not have value for prediction of PJI development. Therefore, the authors do not recommend using this test in presumed aseptic hip or knee revision surgery.