Articles | Volume 9, issue 1
https://doi.org/10.5194/jbji-9-9-2024
https://doi.org/10.5194/jbji-9-9-2024
Original full-length article
 | 
24 Jan 2024
Original full-length article |  | 24 Jan 2024

Multiplex PCR test as an intra-operative diagnostic tool for periprosthetic joint infection in presumed aseptic revision hip and knee arthroplasty: a 1-year follow-up study of 200 cases

Thomas J. A. van Schaik, Petra J. C. Heesterbeek, Job L. C. van Susante, Wim H. C. Rijnen, and Jon H. M. Goosen

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Cited articles

Aamot, H. V., Johnsen, B. O., and Skråmm, I.: Rapid diagnostics of orthopedic implant-associated infections using Unyvero ITI implant and tissue infection application is not optimal for Staphylococcus species identification, BMC Res. Notes., 12, 725, https://doi.org/10.1186/S13104-019-4755-5, 2019. 
Auñón, Á., Coifman, I., Blanco, A., García Cañete, J., Parrón-Cambero, R., and Esteban, J.: Usefulness of a Multiplex PCR Assay for the Diagnosis of Prosthetic Joint Infections in the Routine Setting, Orthop. Surg., 14, 383–388, https://doi.org/10.1111/os.13187, 2022. 
Borde, J. P., Häcker, G. A., Guschl, S., Serr, A., Danner, T., Hübner, J., Burrack-Lange, S., Lüdke, G., Helwig, P., Hauschild, O., and Kern, W. V.: Diagnosis of prosthetic joint infections using UMD-Universal Kit and the automated multiplex-PCR Unyvero i60 ITI® cartridge system: a pilot study, Infection, 43, 551–560, https://doi.org/10.1007/s15010-015-0796-4, 2015. 
Corvec, S., Portillo, M. E., Pasticci, B. M., Borens, O., and Trampuz, A.: Epidemiology and new developments in the diagnosis of prosthetic joint infection, Int. J. Artif. Organs, 35, 923–934, https://doi.org/10.5301/IJAO.5000168, 2012. 
Fernandez-Sampedro, M., Salas-Venero, C., Fariñas-Álvarez, C., Sumillera, M., Pérez-Carro, L., Fakkas-Fernandez, M., Gómez-Román, J., Martínez-Martínez, L., and Fariñas, M. C. M.: Postoperative diagnosis and outcome in patients with revision arthroplasty for aseptic loosening, BMC Infect. Dis., 15, 232, https://doi.org/10.1186/S12879-015-0976-Y, 2015. 
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Short summary
This study evaluated the use of an automated custom-made multiplex PCR (mPCR) test of synovial fluid in presumed aseptic revision hip and knee arthroplasties. Of all 200 patients, none developed a periprosthetic joint infection (PJI) within the first year after surgery. The study showed that a positive mPCR test did not have value for prediction of PJI development. Therefore, the authors do not recommend using this test in presumed aseptic hip or knee revision surgery.