Articles | Volume 6, issue 5
https://doi.org/10.5194/jbji-6-119-2021
© Author(s) 2021. This work is distributed under
the Creative Commons Attribution 4.0 License.
the Creative Commons Attribution 4.0 License.
https://doi.org/10.5194/jbji-6-119-2021
© Author(s) 2021. This work is distributed under
the Creative Commons Attribution 4.0 License.
the Creative Commons Attribution 4.0 License.
Pseudomonas aeruginosa biofilm killing beyond the spacer by antibiotic-loaded calcium sulfate beads: an in vitro study
Jacob R. Brooks
Department of Microbial Infection and Immunity, The Ohio State University, Wexner Medical Center, Columbus, Ohio, USA
Devendra H. Dusane
Department of Microbial Infection and Immunity, The Ohio State University, Wexner Medical Center, Columbus, Ohio, USA
Kelly Moore
Department of Microbial Infection and Immunity, The Ohio State University, Wexner Medical Center, Columbus, Ohio, USA
Tripti Gupta
Department of Microbial Infection and Immunity, The Ohio State University, Wexner Medical Center, Columbus, Ohio, USA
Craig Delury
Biocomposites Ltd., Keele Science Park, Keele, Staffordshire, ST5 5NL, UK
Sean S. Aiken
Biocomposites Ltd., Keele Science Park, Keele, Staffordshire, ST5 5NL, UK
Phillip A. Laycock
Biocomposites Ltd., Keele Science Park, Keele, Staffordshire, ST5 5NL, UK
Anne C. Sullivan
Department of Orthopaedics, The Ohio State University, Wexner Medical Center, Columbus, Ohio, USA
Jeffrey F. Granger
Department of Orthopaedics, The Ohio State University, Wexner Medical Center, Columbus, Ohio, USA
Matthew V. Dipane
Department of Orthopaedic Surgery, David Geffen School of Medicine at UCLA, Santa Monica, California, USA
Edward J. McPherson
Department of Orthopaedic Surgery, David Geffen School of Medicine at UCLA, Santa Monica, California, USA
Paul Stoodley
CORRESPONDING AUTHOR
Department of Microbial Infection and Immunity, The Ohio State University, Wexner Medical Center, Columbus, Ohio, USA
Department of Orthopaedics, The Ohio State University, Wexner Medical Center, Columbus, Ohio, USA
National Centre for Advanced Tribology at Southampton (nCATS), National Biofilm Innovation Centre (NBIC), Department of Mechanical Engineering, University of Southampton, Southampton, UK
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Edward J. McPherson, Jessica A. Jennings, Omar Yunis, Michael A. Harris, Matthew V. Dipane, Nora L. Curtin, Madhav Chowdhry, Andrew J. Wassef, Joel D. Bumgardner, and Scott P. Noel
J. Bone Joint Infect., 7, 117–125, https://doi.org/10.5194/jbji-7-117-2022, https://doi.org/10.5194/jbji-7-117-2022, 2022
Short summary
Short summary
A simulated large joint model has been established to help better understand the elution mechanics of antimicrobial agents and the devices used to deliver them to a large human joint. This model is unique to previously published models in that it more accurately represents the environment of a large human joint. The model was employed to evaluate four brands of commercial medical-grade calcium sulfate. These products were evaluated in both an unaltered state and when mixed with antibiotics.
Edward J. McPherson, Matthew V. Dipane, Madhav Chowdhry, and Andrew J. Wassef
J. Bone Joint Infect., 6, 405–412, https://doi.org/10.5194/jbji-6-405-2021, https://doi.org/10.5194/jbji-6-405-2021, 2021
Short summary
Short summary
The use of calcium sulfate as an antibiotic delivery vehicle is a growing treatment option used by surgeons in the face of infection following joint replacement surgery. Calcium sulfate can be mixed with antibiotics and formed into small beads, which can be delivered during surgery to the local site of infection. We performed a mixing study to formalize mixing methods for individual and combined antibiotics with a synthetic calcium sulfate product. We report our mixing formulas and set times.
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Short summary
Bacterial biofilm infections of artificial knees are treated by mixing antibiotics into bone cement and calcium sulfate beads. We designed a knee size model to show that cement alone killed biofilms on orthopedic materials and prevented their spread close to the cement, but adding beads significantly killed biofilms further away. The model is useful in helping show how both the spread and the amount of antibiotic carrier can kill biofilms that might be in different places in the joint.
Bacterial biofilm infections of artificial knees are treated by mixing antibiotics into bone...