Combined Staining Techniques for Demonstration of <em>Staphylococcus aureus</em> Biofilm in Routine Histopathology

Jensen, Louise Kruse; Henriksen, Nicole Lind; Bjarnsholt, Thomas; Kragh, Kasper Nørskov; Jensen, Henrik Elvang

doi:https://doi.org/10.7150/jbji.22799

Articles | Volume 3, issue 1

Article
Metrics
Related articles

Articles | Volume 3, issue 1

https://doi.org/10.7150/jbji.22799

Articles | Volume 3, issue 1

Article
Metrics
Related articles

Original full-length article

20 Feb 2018

Original full-length article |

| 20 Feb 2018

Combined Staining Techniques for Demonstration of Staphylococcus aureus Biofilm in Routine Histopathology

Louise Kruse Jensen, Nicole Lind Henriksen, Thomas Bjarnsholt, Kasper Nørskov Kragh, and Henrik Elvang Jensen

Louise Kruse Jensen

Department of Veterinary Clinical and Animal Sciences, Ridebanevej 3, 1870 Frederiksberg C, University of Copenhagen, Denmark

Nicole Lind Henriksen

Department of Veterinary Clinical and Animal Sciences, Ridebanevej 3, 1870 Frederiksberg C, University of Copenhagen, Denmark

Thomas Bjarnsholt

Costerton Biofilm Center, Department of Immunology and Microbiology, Blegdamsvej 3B, 2200 Copenhagen N, University of Copenhagen, Denmark

Department of Clinical Microbiology, Juliane Maries Vej 22, 2100 Copenhagen Ø, Copenhagen University Hospital, Denmark

Kasper Nørskov Kragh

Costerton Biofilm Center, Department of Immunology and Microbiology, Blegdamsvej 3B, 2200 Copenhagen N, University of Copenhagen, Denmark

Henrik Elvang Jensen

Department of Veterinary Clinical and Animal Sciences, Ridebanevej 3, 1870 Frederiksberg C, University of Copenhagen, Denmark

Cited articles

Costerton JW, Geesey GG, Cheng K-J. How bacteria stick. Sci Am. 1978;238:86–95.

Donlan RM, Costerton WJ. Biofilms: survival mechanisms of clinically relevant microorganisms. Clin Microbiol Rev. 2002;15:167–193. [PMC free article]

Bjarnsholt T. The role of bacterial biofilms in chronic infections. APMIS. 2013;121:1–51.

Flemming H-C, Wingender J. The biofilm matrix. Nature Rev Microbiol. 2010;8:623–633.

Brady RA, Leid JG, Calhoun JH. et al. Osteomyelitis and the role of biofilms in chronic infection. FEMS Immunol Med Microbiol. 2008;52:13–22.

Hasman H, Moodley A, Guardabassi L. et al. spa type distribution in Staphylococcus aureus originating from pigs, cattle and poultry. Vet Microbiol. 2010;141:326–331.

Aalbæk B, Jensen LK, Jensen HE. et al. Whole-genome sequence of Staphylococcus aureus S54F9 isolated from a chronic disseminated porcine lung abscess and used in human infection models. Genome Announc. 2015;3:1–2. [PMC free article]

Johansen LK, Koch J, Frees D. et al. Pathology and biofilm formation in a porcine model of staphylococcal osteomyelitis. J Comp Path. 2012;147:343–353.

Jensen LK, Koch J, Dich-Jorgensen K, Novel porcine model of implant-associated osteomyelitis: A comprehensive analysis of local, regional and systemic response. J Orthop Res; 2017. doi: 10.1002/jor.23505: 1-11.

Christiansen JG, Jensen HE, Johansen LK. et al. Porcine models of non-bacterial thrombotic endocarditis (NBTE) and infective endocarditis (IE) caused by Staphylococcus aureus: A preliminary study. J Heart Valve Dis. 2013;22:368–376.

Sørensen KE, Skovgaard K, Heegaard PMH. et al. The impact of Staphylococcus aureus concentration on the development of pulmonary lesions and cytokine expression after intravenous inoculation of pigs. Vet Pathol. 2012;49:950–962.

Johansen LK, Koch J, Kirketerp-Møller K. et al. Therapy of haematogenous osteomyelitis - a comparative study in a porcine model and Angolan children. In vivo. 2013;27:305–312.

O'Toole GA. Microtiter dish biofilm formation assay. J Vis Exp; 2011. doi:10.3791/2437: 1-2. [PMC free article]

Malone CL, Boles BR, Lauderdale KJ. et al. Flourescent reporters for Staphylococcus aureus. J Microbiol Methods. 2009;77:251–260. [PMC free article]

Bancroft J.D. & Gamble, M. Theory and practice of histological techniques. New York, USA: Churchill Livingstone Elsevier; 2008. [Google Scholar]

Luna LG. Manual of histologic and methods of the armed forces institute of pathology. New York, USA: McGraw-Hill; 1960. [Google Scholar]

Pearse SGE. Histochemistry theoretical and applied. New York, USA: Churchhill Livingstone; 1985. [Google Scholar]

Lille RD, Fullmer HM. Histopathologic technic and practical histochemistry. New York, USA: McGraw-Hill; 1965. [Google Scholar]

Johansen LK, Frees D, Aalbæk B. et al. A porcine model of acute, haematogenous localized osteomyelitis due to Staphylococcus aureus: a pathomorphological study. APMIS. 2011;119:111–118. [PMC free article]

Yarwood JM, Bartels DJ, Volper EM. et al. Quorum sensing in Staphylococcus aureus biofilms. J Bacteriol. 2004;186:1838–1850. [PMC free article]

Høiby N, Bjarnsholt T, Moser C. et al. ESCMID guideline for the diagnosis and treatment of biofilm infections 2014. Clin Microbiol Infect. 2015;21:S1–S25.

Rumbaugh KP, Carty NL. In vivo models of biofilm infection. In: Bjarnsholt T, editor. Biofilm infections, 1st ed. New York: Springer-Verlag; 2011. pp. 267–290. [Google Scholar]

Winther B, Gross BC, Hendley JO. et al. Location of bacterial biofilm in the mucus overlaying the adenoid by light microscopy. Arch Otolaryngol Head Neck Surg. 2009;135:1239–1245.

Hochstim CJ, Choi JY, Lowe D. et al. Biofilm detection with hematoxylin-eosin staining. Arch Otolaryngol Head Neck Surg. 2010;136:453–456.

Tóth L, Csomor P, Sziklai I. et al. Biofilm detection in chronic rhinosinusitis by combined application of hematoxylin-eosin and gram staining. Eur Arch Otorhinolaryngol. 2011;268:1455–1462.

Harrison-Balestra C, Cazzangia AL, Davis SC. et al. A wound-isolated Pseudomonas aeruginosa grows a biofilm in vitro within 10 hours and is visualized by light microscopy. Dermatol Surg. 2003;29:631–635.

Stoodley P, Nistico L, Johnson S. et al. Direct demonstration of viable Staphylococcus aureus biofilms in an infected total joint arthroplasty. J Bone Joint Surg Am. 2008;90:1751–1758. [PMC free article]

Izano EA, Amarante MA, Kher WB. et al. Differential roles of poly-N-acetylglucosamine surface polysaccharide and extracellular DNA in Staphylococcus aureus and Staphylococcus epidermidis biofilms. Appl Environ Microbiol. 2008;74:470–476. [PMC free article]

Ziran BH. Osteomyelitis. J Trauma. 2007;62:59–60.

Bjarnsholt T, Alhede M, Alhede M. et al. The in vivo biofilm. Trends Microbiol. 2013;21:466–474.

Parsek MR, Singh PK. Bacterial biofilms: an emerging link to disease pathogenesis. Annu Rev Microbiol. 2003;57:677–701.