Journal of Bone and Joint Infection
Journal of Bone and Joint Infection
JBJI
Articles | Volume 10, issue 4
Articles | Volume 10, issue 4
https://doi.org/10.5194/jbji-10-317-2025
© Author(s) 2025. This work is distributed under
the Creative Commons Attribution 4.0 License.
https://doi.org/10.5194/jbji-10-317-2025
© Author(s) 2025. This work is distributed under
the Creative Commons Attribution 4.0 License.
Articles | Volume 10, issue 4
Original full-length article
 | 
21 Aug 2025
Original full-length article |  | 21 Aug 2025

A prospective evaluation of the inoculation of homogenised tissue and bone biopsies in blood culture bottles for the diagnosis of orthopaedic-device-related infections

Ann-Sophie Jacob, Jolien Onsea, Laura Bessems, Pauline Spoormans, Georges Vles, Willem-Jan Metsemakers, Sien Ombelet, and Melissa Depypere

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Cited articles

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Birdsall, J., Tambosis, E., and Siarakas, S.: Isolation of clinically significant microorganisms from prosthetic joint tissue using BacT/ALERT paediatric blood culture bottles compared with solid culture media and enrichment broth, Pathology, 53, 515–519, https://doi.org/10.1016/j.pathol.2020.08.015, 2021. 
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Short summary
Fracture-related infections (FRIs) and periprosthetic joint infections (PJIs) fail to recover pathogens, posing a diagnostic challenge and impacting treatment. This new method involves homogenising biopsies with beads to release bacteria, followed by inoculation in blood culture bottles using an automated system. This technique shows similar sensitivity to conventional methods, with improved specificity and faster pathogen detection. To date, no other studies have reported an optimized method for incubating biopsies from patients with FRIs.
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