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Journal of Bone and Joint Infection An open-access journal of the European Bone and Joint Infection Society and the MusculoSkeletal Infection Society
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Volume 3, issue 1
J. Bone Joint Infect., 3, 43–49, 2018
https://doi.org/10.7150/jbji.22121
© Author(s) 2018. This work is distributed under
the Creative Commons Attribution 4.0 License.
J. Bone Joint Infect., 3, 43–49, 2018
https://doi.org/10.7150/jbji.22121
© Author(s) 2018. This work is distributed under
the Creative Commons Attribution 4.0 License.

Original full-length article 12 Apr 2018

Original full-length article | 12 Apr 2018

Effects of Local Application of Nano-silver on Osteomyelitis and Soft Tissue Infections: An Experimental Study in Rats

Bahattin Kemah1, Gökçer Uzer2, Yalçın Turhan3, Burak Özturan4, Bülent Kılıç5, Bilge Sümbül Gültepe6, Ayşe Bahar Ceyran7, Selim Ertürk8, Burak Aksoylu8, Özlem Şenaydın9, and Korhan Özkan10 Bahattin Kemah et al.
  • 1Orthopedics and Traumatology Department, Ağrı State Hospital, Ağrı, Turkey.
  • 2Orthopedics and Traumatology Department, Bezmialem University Medical Faculty, Istanbul, Turkey.
  • 3Orthopedics and Traumatology Department, Duzce University Medical Faculty, Duzce, Turkey.
  • 4Orthopedics and Traumatology Department, Nizip State Hospital, Gaziantep, Turkey.
  • 5Orthopedics and Traumatology Department, Gelişim University Faculty of Health Sciences, Istanbul, Turkey
  • 6Microbiology Department, Bezmialem University Medical Faculty, Istanbul, Turkey.
  • 7Pathology Department, Medeniyet University Medical Faculty, Istanbul,Turkey.
  • 8Metallurgical Engineering Department, İstanbul Technical University Engineering, Istanbul, Turkey.
  • 9Microbiology Department, Medeniyet University Medical Faculty, Istanbul,Turkey.
  • 10Orthopedics and Traumatology Department, Medeniyet University Medical Faculty, Istanbul,Turkey.

Keywords: Nano-silver, Osteomyelitis, Soft Tissue Infection

Abstract. Purpose: Bone and soft tissue infections are among the least desired complications after orthopaedic surgery. This study analysed the in vivo effects of the local application of nano-silver particles (AgNPs) [1nm = 1 billionth of a meter] in soft tissue infections.

Materials-Method: An experimental osteomyelitis model was formed by inoculating both tibias of 24 rats with methicillin-resistant Staphylococcus aureus. The rats were followed without treatment for 21 days. Blood samples and tibial x-rays at day 21 confirmed the development of infection. Then, the rats were divided randomly into two groups. One group (12 rats) underwent surgical debridement and received 21 days of teicoplanin therapy. The second group had the same treatment, with the addition of local nano-silver. All of the rats were sacrificed at day 42. Blood and wound swab samples were taken and the culture results were analysed.

Results: No differences were observed between the groups in healing values at pathological examination, or in changes in the number of colonies at days 21 and 42. No differences in white blood cell count (WBC) were observed between the groups before and after the treatment.

Conclusion: Although in vitro studies suggest the effectiveness of AgNPs on pathogens, we found that the application of nano-silver did not make any difference when used in addition to the classical osteomyelitis treatment with antibiotics and local surgical debridement. We believe that additional in vivo studies using repeated nano-silver application could be beneficial.

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