<p class="p p-first" id="__p2"><strong>Introduction:</strong> Prosthetic joint infection (PJI) due to <em>Streptococcus bovis</em> group (SBG), specifically<em> S. bovis</em> biotype I (<em>S. gallolyticus</em>), is rare and associated with colorectal carcinoma. Little has been published regarding <em>SBG</em> PJI. We analyzed nine cases of SBG PJI at our institution, the largest series to date.</p><p id="__p3"><strong>Methods</strong>: The medical records of patients diagnosed with SBG PJI between 2000-2017 were reviewed. Patients were followed until death, failure, or loss to follow-up. Mean follow-up was 37 months (range 0.5-74 months).</p><p id="__p4"><strong>Results:</strong> Nine PJI in 8 patients with mean prosthesis age at diagnosis of 8 years (range 4 weeks-17 years) were identified. The median duration between symptom onset and treatment was 38 weeks (range 0.3 weeks-175 weeks). 8/9 had their PJI eradicated with treatment based on acuity of symptoms. Acute PJI (2) was treated with DAIR, and chronic PJI (7) was treated with 2-stage revision arthroplasty. 1 PJI with chronic PJI developed recurrent infection after initial treatment. All patients received post-operative IV antibiotics. 7/8 patients received Ceftriaxone. Three patients received lifelong oral antibiotics. 7/8 patients underwent colonoscopy. 5/7 patients were found to have polyps following PJI diagnosis with one carcinoma and two dysplastic polyps. The two patients without polyps had identifiable gastrointestinal (GI) mucosal abnormality: tooth extraction prior to symptom onset and diverticulosis on chronic anticoagulation.</p><p class="p p-last" id="__p5"><strong>Conclusion:</strong> SBG PJI is typically due to hematologic seeding. Colonoscopy should be pursued for patients with SBG PJI. Surgical treatment dictated by infection acuity and 6-week course of Ceftriaxone seems sufficient to control infection.</p>